SERVICES
 

Generation of transgenic mice by injection of DNA in fertilized oocytes.

The platform also provides help in the design of the DNA constructs and shares available plasmids.

Homologous recombination

Platform users take charge of generating DNA constructs. Culture, electroporation and selction of ES cells is performed by platform users under supervision of Dr. Achouri. The platform takes charge of injecting the recombined ES cells in blastocysts and reimplanting th einjected blastocysts.

CRISPR-CAS9 technology

This technology is currently being set up.

Embryo transfer

In order to guarantee high quality sanitary status the platform can transfer embryos from infected females into pathogen-free pseudo-pregnant females. Platform users have to provide pregnant females.

Embryos cryopreservation

Transgenic lines are stored as vitrified embryos or frozen sperm. Users provide the platform with pregnant females or with males whose fertility has been validated.