CLEANING AND COATING COVERSLIPS

Cleaning coverslips

1. Sonicate 3 times 5 min in 10% Acetic Acid in 95% Alcohol

2. Sonicate 2 times 5 min in 95% Alcohol

3. Stock in 95% Alcohol at 4°C

Before use on cultures : rinse 2 times in sterile water and once in sterile PBS

Coating coverslips with Poly-L-lysine

            (just before cell culture )

1. Cleaning:   - Sonicate 3 times 5 min in 10% Acetic Acid in 95% Alcohol

                      - Stock coverlips in 95 % Alcohol until use (Sterile)

2. Before use : Sonicate 2 times 5 min in sterile water

3. Coating : 50 µl of  a 1 mg/ml Poly-L-Lysine sterile solution per coverslip for 30 min

4. Wash 5 times 5 min in sterile water

5. Wash 2 times 5 min in culture medium