PBS, PBS-Ca2+ and Phosphate buffer
| PBS | (pH 7.4) NaCl KCl Na2HPO4 (anhydr.) KH2PO4 (anhydr.) |
1 L 8 g 0.2 g 1.15 g 0.2 g |
5 L 40 g 1 g 5.75 g 1 g |
| PBS-Ca2+ | (pH 7.0) NaCl KCl Na2HPO4 (anhydr.) KH2PO4 (anhydr.) CaCl2.2H2O MgSO4.7H2O |
1 L 8 g 0.4 g 0.06 g 0.06 g 0.53 g 0.74 g |
5 L 40 g 2 g 0.3 g 0.3 g 2.64 g 3.7 g |
0.2M Phosphate buffer stock solution
Solution 1 : 28.5 g Na2HPO4.2H2O in 800 ml water
Solution 2 : 6.2 g KH2PO4(anhydr.) in 250 ml water
Add solution 2 to 1 until pH is 7.4
