USEFUL TOOLS

1. CHARACTERISTICS OF FLUOROCHROMES AND FILTERS 

•  http://www.lifetechnologies.com/be/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html
•  http://rsb.info.nih.gov/nih-image/more-docs/confocals.html
•  https://www.micro-shop.zeiss.com/?s=13295788143eed4&l=nl&p=be&f=f
•  http://www.microscopyu.com/articles/fluorescence/filtercubes/filterindex.html

2. BOOKS

• Methods in Cell Biology. Vol 70. Cell Biological Applications of Confocal Microscopy. Second Edition. Edited by Brian Matsumoto. Academic Press – 2002.
• Methods in Molecular Biology. Vol 122. Confocal Microscopy Methods and Protocols. Edited by Stephen W. Paddock. Humana Press – 1999
• The Methods Express series. Cell Imaging. Edited by D. Stephens. Scion – 2006
• Confocal and Two-Photon Microscopy. Foundations, Applications and Advances. Edited by Alberto Diaspro. Wiley-Liss – 2002

3. PROTOCOLS

• PBS, PBS-Ca2+ and Phosphate buffer
• Labeling of cells with Phalloidine-Rhodamine
• Cleaning and Coating coverslips
• Fixatives
  • Preparation of 4% Formaldehyde in 0.1 M Phosphate buffer
  • Standard perfusion fixation
  • Perfusion fixation for immunolocalization
  • Cell cultures
• Immunofluorescence on cells in culture (1)
• Immunofluorescence on cells in culture (2)
• HRP -  DAB on cells in culture